Prophenoloxidase Activation Pathway and its Role in Freshwater Crab, Barytelphusa Cunicularis (West-wood) Immunity

نویسندگان

  • Sara Jones
  • A K Khan
چکیده

The prophenoloxidase activating system (proPO-AS) in fresh water crab, Barytelphusa cunicularis is localized in the hemocytes. Using anticoagulant (EDTA-Citrate buffer) it was possible to isolate intact hemocytes without causing extensive degranulation & subsequent clotting. Activation and conversion of prophenoloxidase to phenoloxidase(PO) was carried out by serine proteinase. PO was found to have molecular range between 60-70kDa. PO showed increased activity in the presence of elicitors such as β-1, 3 glucan, SDS & Calcium. The activation was dependent upon Ca ions and occurs with limited proteolysis. ß-1, 3 glucan, SDS and Ca activated the peptidase in HLS. Active PO strongly bound to foreign surfaces and this phenomenon may assist in providing opsonic properties for the proPO cascade.

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تاریخ انتشار 2010